Journal: Cell Death & Disease

Article Title: Metalloproteinase 1 downregulation in neurofibromatosis 1: Therapeutic potential of antimalarial hydroxychloroquine and chloroquine

doi: 10.1038/s41419-021-03802-9

Figure Lengend Snippet: Biopsy samples of cutaneous tumor were taken from the dermis of an NF1 patient and subjected to hematoxylin and eosin ( a , e , i ), S100 ( b , f , j ), Azan–Mallory ( c , g , k ), and MMP1 staining ( d , h , l ). Representative images of the tumor are shown in a – d , and magnified images of the squares in a – d are shown in i – l . The arrowheads show the early lesions of the peripheral regions, and their magnified images are shown in e – h . m , n Cutaneous biopsy samples of normal skin, perilesional normal skin, and tumor lesion were taken from five NF1 patients, on which MMP1 staining was performed. Arrowhead: MMP1-expressing cells. VW: blood vessel wall. n MMP1-positive cells were counted from three images of each section (normal skin, perilesional normal skin, and tumor); mean (with SEM) percentage of positive cells from five patients is shown ( n = 5).

Article Snippet: For immunohistochemical analysis, anti-collagen antibody (Nichirei Biosciences Inc.), anti-MMP1 antibody (Nichirei Biosciences Inc.), anti-S100 protein antibody (Nichirei Biosciences Inc.), AP-linked anti-rabbit antibody (Abcam), and rabbit isotype control antibody (BioLegend, San Diego, CA, USA) were used.

Techniques: Staining, Expressing

Journal: Cell Death & Disease

Article Title: Metalloproteinase 1 downregulation in neurofibromatosis 1: Therapeutic potential of antimalarial hydroxychloroquine and chloroquine

doi: 10.1038/s41419-021-03802-9

Figure Lengend Snippet: Three cell lines of each of HEFs and NFFs were cultured in triplicate wells for 48 h. a Relative mRNA expression of the indicated genes is shown. Black bars represent expression in HEFs and white bars represent expression in NFFs. b , c Expression of the indicated proteins was analyzed by western blot. Representative images of western blot are shown in b and relative expression normalized to β-actin is shown in c . d MMP1 production for 24 h was measured. All cell lines were cultured in triplicate wells and the mean ± SEM of HEFs or NFFs is shown.

Article Snippet: For immunohistochemical analysis, anti-collagen antibody (Nichirei Biosciences Inc.), anti-MMP1 antibody (Nichirei Biosciences Inc.), anti-S100 protein antibody (Nichirei Biosciences Inc.), AP-linked anti-rabbit antibody (Abcam), and rabbit isotype control antibody (BioLegend, San Diego, CA, USA) were used.

Techniques: Cell Culture, Expressing, Western Blot

Journal: Cell Death & Disease

Article Title: Metalloproteinase 1 downregulation in neurofibromatosis 1: Therapeutic potential of antimalarial hydroxychloroquine and chloroquine

doi: 10.1038/s41419-021-03802-9

Figure Lengend Snippet: Three cell lines of each of HEFs and NFFs were cultured in triplicate wells and treated with 50 μM CQ, 50 μM HCQ, 5 nM BafA, or vehicle. a Representative cell morphology of NFFs treated with the indicated drugs or vehicle. Arrowheads indicate vesicles. Scale bar indicates 1 μm. b Relative MMP1 mRNA expression in 24 h of treatment is shown. c MMP1 proteins in culture medium upon 48 h of treatment with lysosomotropic agents were analyzed by ELISA. Black bar indicates expression in HEFs and white bar indicates expression in NFFs ( b , c ). The data represented the mean ± SEM of the three independent experiments.

Article Snippet: For immunohistochemical analysis, anti-collagen antibody (Nichirei Biosciences Inc.), anti-MMP1 antibody (Nichirei Biosciences Inc.), anti-S100 protein antibody (Nichirei Biosciences Inc.), AP-linked anti-rabbit antibody (Abcam), and rabbit isotype control antibody (BioLegend, San Diego, CA, USA) were used.

Techniques: Cell Culture, Expressing, Enzyme-linked Immunosorbent Assay

Journal: Cell Death & Disease

Article Title: Metalloproteinase 1 downregulation in neurofibromatosis 1: Therapeutic potential of antimalarial hydroxychloroquine and chloroquine

doi: 10.1038/s41419-021-03802-9

Figure Lengend Snippet: a , b Three cell lines of each HEFs and NFFs were treated with 50 μM CQ, 50 μM HCQ, or 5 nM BafA for 48 h and NF1 expression was analyzed by western blotting. Representative images from three independent experiments are shown in a . NF1 expression was normalized with β-actin and relative expression levels were calculated considering the intensity of vehicle-treated sample as 1 and shown in b . The data represented the mean ± SEM of the three independent experiments. c , d The HEFs (KYU168) and NFFs (KYU101) were transfected with scrambled RNA or si_NF1, and treated with 50 μM HCQ. c NF1 and MMP1 mRNA expression in 24 h is shown. The data represented the mean ± SD of the three independent experiments. d NF1, MMP1, and β-actin expression was analyzed by western blot and representative images from three independent experiments are shown in the upper part. Relative MMP1 expression is shown in the lower part. The data represented the mean ± SD of the three independent experiments. *: compared among drug-treated groups. †: compared with scrambled RNA-transfected group.

Article Snippet: For immunohistochemical analysis, anti-collagen antibody (Nichirei Biosciences Inc.), anti-MMP1 antibody (Nichirei Biosciences Inc.), anti-S100 protein antibody (Nichirei Biosciences Inc.), AP-linked anti-rabbit antibody (Abcam), and rabbit isotype control antibody (BioLegend, San Diego, CA, USA) were used.

Techniques: Expressing, Western Blot, Transfection

Journal: Cell Death & Disease

Article Title: Metalloproteinase 1 downregulation in neurofibromatosis 1: Therapeutic potential of antimalarial hydroxychloroquine and chloroquine

doi: 10.1038/s41419-021-03802-9

Figure Lengend Snippet: a HEFs were treated with 50 μM HCQ for 6 h or 100 nM FICZ for 1 h and stained with anti-AHR antibody. Nuclei were stained with 4,6-diamidino-2-phenylindole (DAPI). Scale bar, 50 μm. b – d HEFs (KYU168) and NFFs (KYU101) were transfected with scrambled RNA or si_AHR and treated with 50 μM HCQ. b CYP1B1 mRNA expression in 24 h is shown. c AHR and MMP1 mRNA expression in 24 h is shown. The data represented the mean ± SD of the three independent experiments ( b , c ). d NF1, AHR, MMP1, and β-actin expression was analyzed by western blot and representative images from three independent experiments are shown. * compared among drug-treated groups. † compared with scrambled RNA-transfected group.

Article Snippet: For immunohistochemical analysis, anti-collagen antibody (Nichirei Biosciences Inc.), anti-MMP1 antibody (Nichirei Biosciences Inc.), anti-S100 protein antibody (Nichirei Biosciences Inc.), AP-linked anti-rabbit antibody (Abcam), and rabbit isotype control antibody (BioLegend, San Diego, CA, USA) were used.

Techniques: Staining, Transfection, Expressing, Western Blot

Journal: Cell Death & Disease

Article Title: Metalloproteinase 1 downregulation in neurofibromatosis 1: Therapeutic potential of antimalarial hydroxychloroquine and chloroquine

doi: 10.1038/s41419-021-03802-9

Figure Lengend Snippet: HEFs (KYU168) and NFFs (KYU101) were treated with 50 μM HCQ and phosphorylation inhibitors of the MAPK pathway for 24 h; MMP1 mRNA ( a ) and protein ( b ) expression is shown. PD: 10 μM PD184352 (ERK inhibitor), AKTI: 5 μM (AKT inhibitor), SB: 10 μM SB203580 (p38 MAPK inhibitor), and SP: 10 μM SP600125 (JNK inhibitor). The data represented the mean ± SD of the three independent experiments ( a ). The different letters (a, b, c) above the bars indicate statistically significant differences ( P < 0.05).

Article Snippet: For immunohistochemical analysis, anti-collagen antibody (Nichirei Biosciences Inc.), anti-MMP1 antibody (Nichirei Biosciences Inc.), anti-S100 protein antibody (Nichirei Biosciences Inc.), AP-linked anti-rabbit antibody (Abcam), and rabbit isotype control antibody (BioLegend, San Diego, CA, USA) were used.

Techniques: Expressing

Journal: Cell Death & Disease

Article Title: Metalloproteinase 1 downregulation in neurofibromatosis 1: Therapeutic potential of antimalarial hydroxychloroquine and chloroquine

doi: 10.1038/s41419-021-03802-9

Figure Lengend Snippet: Schematic summarizing of MMP1 induction by HCQ and CQ in NFFs. a , b MMP1 reduction reads collagen accumulation and generates or worsen neurofibromas in neurofibromatosis 1 patients. c AHR activated by HCQ or CQ induces ERK phosphorylation and MMP1 expression. At the same time, HCQ and CQ inhibit lysosomal degradation of MMP1.

Article Snippet: For immunohistochemical analysis, anti-collagen antibody (Nichirei Biosciences Inc.), anti-MMP1 antibody (Nichirei Biosciences Inc.), anti-S100 protein antibody (Nichirei Biosciences Inc.), AP-linked anti-rabbit antibody (Abcam), and rabbit isotype control antibody (BioLegend, San Diego, CA, USA) were used.

Techniques: Expressing

Journal: Oncotarget

Article Title: Combination therapy of chitosan, gynostemma, and motherwort alleviates the progression of experimental rat chronic renal failure by inhibiting STAT1 activation

doi: 10.18632/oncotarget.24125

Figure Lengend Snippet: (a) Western blots. (b) PAI-1 expression. (c) Collagen I expression. (d) MMP-1 expression. (e) TIMP-1 expression. The intensity of the ECM mediators was normalized to β-actin. The expression of protein markers in Ctrl was set to 1, and used to normalize the expression of other markers in treatment groups. * P< 0.05, ** P< 0.01.

Article Snippet: Monoclonal anti-collagen I, anti-TIMP-1 and anti-matrix metalloprotease-1(MMP-1) were obtained from Signalway (Pirland, Texas, USA), and monoclonal anti-plasminogen activator inhibitor 1(PAI-1) was from KeyGEN Ltd., (Nanjing, Jiangsu, China).

Techniques: Western Blot, Expressing

Journal: Scientific Reports

Article Title: Plasma matrix metalloproteinase 1 improves the detection and survival prediction of esophageal squamous cell carcinoma

doi: 10.1038/srep30057

Figure Lengend Snippet: Seven genes were identified to have a fold-change >1.5 and a p-value < 0.005. Their importance on discriminating ESCC from normal tissues was evaluated by the mean decrease in Gini index (MDG) obtained from the Random Forests algorithm. Among the 7 genes, MMP1 ranked number four according to the MDG value.

Article Snippet: Immunohistochemistry (IHC) study was performed on the formalin-fixed paraffin-embedded ESCC tissues according to the manufacturer’s instruction using anti-MMP1 antibody (Merck; MAB-3307; 1:300 dilution) and anti-mouse/rabbit secondary antibody conjugated with HRP (ChemMate DAKO EnVision Detection Kit, Code: K5007, DAKO).

Techniques:

Journal: Scientific Reports

Article Title: Plasma matrix metalloproteinase 1 improves the detection and survival prediction of esophageal squamous cell carcinoma

doi: 10.1038/srep30057

Figure Lengend Snippet: Distribution of plasma MMP1 levels and demographic characteristics in ESCC patients and controls.

Article Snippet: Immunohistochemistry (IHC) study was performed on the formalin-fixed paraffin-embedded ESCC tissues according to the manufacturer’s instruction using anti-MMP1 antibody (Merck; MAB-3307; 1:300 dilution) and anti-mouse/rabbit secondary antibody conjugated with HRP (ChemMate DAKO EnVision Detection Kit, Code: K5007, DAKO).

Techniques:

Journal: Scientific Reports

Article Title: Plasma matrix metalloproteinase 1 improves the detection and survival prediction of esophageal squamous cell carcinoma

doi: 10.1038/srep30057

Figure Lengend Snippet: ( A ) The AUROC of MMP1 alone was 0.557; the optimal cut-off value was very close to the highest quartile of all study subjects (9.67 ng/mL). ( B ) Among subjects with any one of the risk factors, adding plasma MMP1 significantly increased the AUROC by 0.0361 (p = 0.0013). ( C ) Among subjects with any two of the risk factors, adding plasma MMP1 significantly increased the AUROC by 0.0175 (p = 0.0097). ( D ) Among subjects with all three risk factors, adding plasma MMP1 increased the AUROC by 0.0225 (p = 0.0676).

Article Snippet: Immunohistochemistry (IHC) study was performed on the formalin-fixed paraffin-embedded ESCC tissues according to the manufacturer’s instruction using anti-MMP1 antibody (Merck; MAB-3307; 1:300 dilution) and anti-mouse/rabbit secondary antibody conjugated with HRP (ChemMate DAKO EnVision Detection Kit, Code: K5007, DAKO).

Techniques:

Journal: Scientific Reports

Article Title: Plasma matrix metalloproteinase 1 improves the detection and survival prediction of esophageal squamous cell carcinoma

doi: 10.1038/srep30057

Figure Lengend Snippet: Association between plasma MMP1 concentrations and ESCC risk.

Article Snippet: Immunohistochemistry (IHC) study was performed on the formalin-fixed paraffin-embedded ESCC tissues according to the manufacturer’s instruction using anti-MMP1 antibody (Merck; MAB-3307; 1:300 dilution) and anti-mouse/rabbit secondary antibody conjugated with HRP (ChemMate DAKO EnVision Detection Kit, Code: K5007, DAKO).

Techniques:

Journal: Scientific Reports

Article Title: Plasma matrix metalloproteinase 1 improves the detection and survival prediction of esophageal squamous cell carcinoma

doi: 10.1038/srep30057

Figure Lengend Snippet: ( A ) There was a borderline significant difference of AUROC after adding plasma MMP1 to alcohol (difference = 0.0155, p = 0.0719). ( B ) There was a borderline significant difference of AUROC after adding plasma MMP1 to betel nut (difference = 0.0210, p = 0.0629). ( C ) adding plasma MMP1 to smoking habit significantly increased the AUROC by 0.0327 (p < 0.0001). ( D ) After considering all three risk factors, adding plasma MMP1 did not improve discrimination.

Article Snippet: Immunohistochemistry (IHC) study was performed on the formalin-fixed paraffin-embedded ESCC tissues according to the manufacturer’s instruction using anti-MMP1 antibody (Merck; MAB-3307; 1:300 dilution) and anti-mouse/rabbit secondary antibody conjugated with HRP (ChemMate DAKO EnVision Detection Kit, Code: K5007, DAKO).

Techniques:

Journal: Scientific Reports

Article Title: Plasma matrix metalloproteinase 1 improves the detection and survival prediction of esophageal squamous cell carcinoma

doi: 10.1038/srep30057

Figure Lengend Snippet: Patients with plasma MMP1 > 9.67 ng/mL had significantly shorter survival compared to those with lower MMP1 levels (p = 0.0265). Thirty-four patients were excluded for survival analysis because they died (N = 15) or lost follow-up (N = 19) within 1 month of cancer diagnosis.

Article Snippet: Immunohistochemistry (IHC) study was performed on the formalin-fixed paraffin-embedded ESCC tissues according to the manufacturer’s instruction using anti-MMP1 antibody (Merck; MAB-3307; 1:300 dilution) and anti-mouse/rabbit secondary antibody conjugated with HRP (ChemMate DAKO EnVision Detection Kit, Code: K5007, DAKO).

Techniques:

Journal: Scientific Reports

Article Title: Plasma matrix metalloproteinase 1 improves the detection and survival prediction of esophageal squamous cell carcinoma

doi: 10.1038/srep30057

Figure Lengend Snippet: Relationship of plasma MMP1 with the survival of ESCC patients in Cox regression models (N = 176).

Article Snippet: Immunohistochemistry (IHC) study was performed on the formalin-fixed paraffin-embedded ESCC tissues according to the manufacturer’s instruction using anti-MMP1 antibody (Merck; MAB-3307; 1:300 dilution) and anti-mouse/rabbit secondary antibody conjugated with HRP (ChemMate DAKO EnVision Detection Kit, Code: K5007, DAKO).

Techniques:

Journal:

Article Title: Expression and distribution of matrix metalloproteinases and their inhibitors in the human iris and ciliary body

doi:

Figure Lengend Snippet: Distribution and intensity of immunoreactivity for MMPs and TIMPs in the iris. Grading is presented as medians and interquartile ranges

Article Snippet: Mouse anti-human antibodies to individual MMPs (MMP-1, 2, 3, 9) and TIMPs 1–4 were obtained from ICN Biomedicals, Australia.

Techniques:

Journal:

Article Title: Expression and distribution of matrix metalloproteinases and their inhibitors in the human iris and ciliary body

doi:

Figure Lengend Snippet: Distribution and intensity of immunoreactivity for MMPs and TIMPs in the ciliary body

Article Snippet: Mouse anti-human antibodies to individual MMPs (MMP-1, 2, 3, 9) and TIMPs 1–4 were obtained from ICN Biomedicals, Australia.

Techniques:

Journal: Oncotarget

Article Title: MMP-1 promoter polymorphism is associated with risk of radiation-induced lung injury in lung cancer patients treated with radiotherapy

doi: 10.18632/oncotarget.12164

Figure Lengend Snippet: ( A ) Representative photomicrograph of pneumocytes with rs1144393 AA or AG/GG genotypes. Original magnification, 20×. ( B ) IHC quantification data for MMP-1 expression in lung tissues from patients with the rs1144393 AA genotype ( n = 22) and the rs1144393 AG or GG genotypes ( n = 6) were evaluated by the mean optical density per pixel obtained from alveolar areas. Significant differences were identified using the Mann–Whitney U test ( p = 0.022), data are represented as mean ± SEM.

Article Snippet: Deparaffinized sections were subjected to antigen retrieval and then incubated with 3% hydrogen peroxide for 15 min. After blocking with 5% bovine serum albumin, the sections were incubated with anti-MMP-1 antibody (AP11874c,1:50, Abgent, San Diego, CA, USA) overnight at 4°C, followed by incubation with secondary goat anti-rabbit antibody (GB23303; Wuhan Goodbio Technology Co., Wuhan, China) for 50 min. Counterstaining with hematoxylin was performed, and 3,3′-diaminobenzidine positivity was analyzed.

Techniques: Expressing, MANN-WHITNEY

Journal: Oncotarget

Article Title: MMP-1 promoter polymorphism is associated with risk of radiation-induced lung injury in lung cancer patients treated with radiotherapy

doi: 10.18632/oncotarget.12164

Figure Lengend Snippet: ( A ) Comparison of plasma MMP-1 concentrations between patients with the rs1144393 AA genotype ( n = 32) and those with the AG or GG genotypes ( n = 8; p = 0.018). ( B ) Comparison of plasma MMP-1 levels between patients displaying grade < 2 RILI ( n = 19) and those displaying grade ≥ 2 RILI ( n = 21; p = 0.014). Significant differences were determined using the Mann–Whitney U test, data are represented as mean ± SEM.

Article Snippet: Deparaffinized sections were subjected to antigen retrieval and then incubated with 3% hydrogen peroxide for 15 min. After blocking with 5% bovine serum albumin, the sections were incubated with anti-MMP-1 antibody (AP11874c,1:50, Abgent, San Diego, CA, USA) overnight at 4°C, followed by incubation with secondary goat anti-rabbit antibody (GB23303; Wuhan Goodbio Technology Co., Wuhan, China) for 50 min. Counterstaining with hematoxylin was performed, and 3,3′-diaminobenzidine positivity was analyzed.

Techniques: MANN-WHITNEY